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| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
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Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
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| Bicalho,K.A.; Passos,L.M.F; Ribeiro,M.F.B. |
| The objective of the present study was to evaluate parasitemia and packed cell volume patterns of dogs experimentally inoculated with two isolates of Babesia canis: one from Belo Horizonte (BH) and the other from Lavras (Lv), Minas Gerais State, Brazil. Both isolates showed similar patterns, with the peak of parasitemia occurring three days post-infection. From the fourth day, parasitemia was detected in low levels (0.01%) with small periodical increases. The packed cell volume decreased after the parasitemia beginning, with oscillations during the experimental period. All dogs remained apparently normal, except one, which had been inoculated with the BH isolate and presented classical clinical signs of babesiosis (weakness, anemia, hemoglobinuria and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cão; Babesia canis; Infecção experimental. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000500016 |
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| Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan. |
| Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1... |
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Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420 |
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